Solve the Empty/Full Capsid Separation Challenge
Gene therapy's journey relies on a precise vehicle: the AAV capsid. But each batch is a mixed convoy, where empty particles travel alongside their DNA-filled counterparts. Arriving at their destination with the wrong cargo can determine the success of the entire mission.
The critical checkpoint in this journey is purification—specifically, the separation of full from empty capsids. This application note details a strategic route through this checkpoint, using the principle of anion exchange chromatography. It’s a serotype-independent map, guiding you to optimize buffer conditions and elution gradients to achieve clear resolution.
The most compelling part of the story is its accessibility: this optimized separation can be executed without a complex LC system, using a simple syringe-driven workflow. Proven with data across AAV2, AAV5, and AAV8 serotypes, this strategy turns a major analytical challenge into a routine, equipment-free process.
Discover how to bring clarity and efficiency to your AAV purification pipeline. Download the application note to explore the data and implement the method.